Worm apparatus. To condition worms you will need a conditioning tray, paper towel, butanol, rose oil, dechlorinated water, CS syringe, US syringe, and gloves

To begin, cut the paper towel in the shape of the conditioning tray.

Moisten the paper towel with dechlorinated water and place in the conditioning tray. This is necessary to keep the worm moist during conditioning.

Remove the earthworm from its container and using gloves wash the worm with dechlorinated water. The gloves are necessary when handling the worm because of impurities on the hands of the experimenter.

Expose earthworm to a CS of rose oil closely followed by a US of butanol. The CS-US interval should be as short as possible (approx.1-2 seconds). The intertrial interval is 1 minute

Unconditioned response, worm will retract when exposed to butanol.

Conditioned response to rose oil.